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UPLC-MS extracted ion chromatogram of m/z 854.0789 [M+3H]3+ of tryptic digest from mAb1 spiked with a synthetic peptide containing alanine to serine sequence variant (identified as peak 2). The other identified peaks (1a , 1b and 4) correspond to peptides containing isomers of oxidized tryptophan at position 10 in the peptide, and the potential lysine to hydroxylysine modification at the C-terminus (peak 3).
