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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Open Life Sci. 2015 Oct 19;10(1):461–478. doi: 10.1515/biol-2015-0048

Figure 1. Generation of Cre-inducible Rosa26-myr-p110α knock-in allele.

Figure 1

(A) Schematic diagrams of the expression construct cloned into pRosa26 targeting vector, a portion of Rosa26 locus with restriction sites, the resulting targeted locus, and the myr-p110α allele following Cre-mediated excision. Labeled primers for PCR genotyping are indicated with blue arrows. The probe used for Southern blot analysis is shown as a light green box in the Rosa26 locus. (B) Representative result of PCR detecting 602 bp of wild-type (WT) band and 314 bp of integrated construct band. (C) Genomic Southern blotting with a 593 bp of DIG-labeled DNA probe, detecting either 15.6 kb of WT allele and 4.3 kb of targeted allele.