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. 2016 Jul 29;16:260. doi: 10.1186/s12906-016-1243-5

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Western Blot analysis: Group I: Control; Group II: MNU; Group III: β-sitosterol10 mg/kg; Group IV: β-sitosterol 20 mg/kg. The western blot analysis containing whole tissue lysate from rat mammary gland was probed for P-gp 9.5 antibody; its immunoreactivity was evident as a band of molecular weight of 27 kDa. In case of control the expression is low in comparison with the toxicant. The low and high dose of β-sitosterol shows a similar pattern of elevated expression in a dose dependent manner. Results of the β-actin analysis are shown as an internal control