Table 4.
Anti-hemolytic activity of A. hydaspica methanol extract and its soluble fractions against H2O2 induced hemolysis
| Samples | Positive control (A) | Negative control (B) | Optical densityC at 560 nm concentration (μg/ml) | % Inhibition of hemolysis at 1000 (μg/ml) | |||
|---|---|---|---|---|---|---|---|
| 100 | 250 | 500 | 1000 | ||||
| AHM | 1.45 ± 0.02 | 0.11 ± 0.05 | 0.69 ± 0.01 | 0.85 ± 0.05 | 0.96 ± 0.05 | 1.15 ± 0.01 | 79.3c |
| AHH | 1.45 ± 0.03 | 0.11 ± 0.05 | 0.65 ± 0.05 | 0.71 ± 0.05 | 0.88 ± 0.05 | 0.98 ± 0.05 | 67.6d |
| AHC | 1.45 ± 0.05 | 0.11 ± 0.05 | 0.51 ± 0.05 | 0.63 ± 0.03 | 0.71 ± 0.05 | 0.87 ± 0.02 | 60.0e |
| AHE | 1.45 ± 0.06 | 0.11 ± 0.05 | 0.72 ± 0.04 | 0.89 ± 0.09 | 1.14 ± 0.01 | 1.42 ± 0.02 | 97.9a |
| AHB | 1.45 ± 0.04 | 0.11 ± 0.05 | 0.67 ± 0.05 | 0.81 ± 0.05 | 0.92 ± 0.05 | 1.29 ± 0.03 | 88.9b |
| AHA | 1.45 ± 0.07 | 0.11 ± 0.05 | 0.49 ± 0.05 | 0.59 ± 0.05 | 0.64 ± 0.05 | 0.79 ± 0.05 | 54.5f |
| QR | 1.45 ± 0.08 | 0.11 ± 0.05 | 0.72 ± 0.04 | 0.89 ± 0.09 | 1.14 ± 0.01 | 1.43 ± 0.02 | 98.6a |
Each value is represented as mean ± SEM (n = 3). Values in same column followed by different letter (a–f) are significantly different (p <0.05), A; phosphate buffer saline replaced extract/fraction as this treatment results in 0 % hemolysis, B; hydrogen peroxide replaced extract to serve as a negative control since this treatment results in 100 % hemolysis, c; lower values are associated with increased cell lysis. AHM A. hydaspica methanol extract, AHH A. hydaspica nhexane fraction, AHE A. hydaspica ethyl-acetate. (Completely Randomized AOV followed by Tukey HSD All-Pairwise Comparison Test)