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. 2016 Jul 29;16:168. doi: 10.1186/s12870-016-0857-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Phenotype of plants. a Pale-green sectors in leaves of T1 plants containing the iDR intermediate following release of selection. Sectors are linked to iDR recombination and provide a visual illustration of the process. b T2 Seedlings from sectoring T1 plants germinated on spectinomycin medium. White aadA-free seedling is arrowed. c A white aadA-free seedling transferred to antibiotic free medium produces new pale-green leaves. d A marker-free T2 plant containing the edited cp genome exhibits a pale-green phenotype compared to (e), a WT plant