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. 2016 Jul 29;16:168. doi: 10.1186/s12870-016-0857-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — GUS accumulation in transplastomic plants with iDR intermediate and edited cp genomes. Blots of total leaf protein from the indicated lines incubated with a GUS-specific antibody (a-b). Diagrams show no translation of the upstream overlapping rbcL sequence, located between the gusA start and rbcL UAG termination codons, in (a), and translation of this sequence in (b). Dilutions of a positive control (GUS+) transplastomic plant accumulating GUS to 5 % of total leaf protein were used to estimate relative amounts (c). MW size standards and band sizes are indicated. GUS activities in leaf extracts (d). Average of three biological replicates showing one standard deviation. 4-methylumbelliferone (4-MU), Fresh weight (FW)