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. 2016 Feb 26;8(3):524–535. doi: 10.1080/19420862.2016.1149662

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This article not subject to US copyright law. Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 4. — Glycan profiling of Fabs and Fcs. The Fabs and Fcs of rituximab (A) and cetuximab (B) were prepared as described in Materials and Methods. Purified Fab and Fc were analyzed by reducing SDS-PAGE (left panel) and lectin microarray (right panel). As noted, the dimeric Fcs (˜ 55 kDa) were reduced to monomeric products (˜ 30 kDa) on SDS-PAGE under reducing conditions.