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. 2015 Dec 29;8(3):468–477. doi: 10.1080/19420862.2015.1134409

Figure 1.

Figure 1.

Characterization of 21 human anti-S mAbs to identify those with high affinity for the S protein. (A) ELISA method. Purified S protein (red) or anti-human Fab antibody (blue) were coated on 96-well plates, and incubated with human Fab antibodies. Bound antibodies were revealed by HRP-conjugated anti-human Fab. (B) SPR method. HBIgG12 and HBIgG21 at various concentrations were applied to BIAcore 3000 sensor chip CM5 immobilized with S protein. Each measurement represents an average of 3 independent assays.