Figure 3.

LOB12.3 variable domain sequences described in this work. LOB12.3-Lv1/Hv1 represent the initial assembly of proteomic data into light and heavy V-domain sequences, where X represents ambiguous regions of sequence (gray highlighting = 113 Da; green = 114 Da). Alignment of this draft to closest V, D and J translated germline gene segments is shown below. LOB12.3-Lv2/Hv2 were refined draft sequences, as defined in Table 1, and ambiguous regions in this light/heavy pair were programmed into a Fab phage display library. Further refinement after phage display led to LOB12.3-Lv3/Hv3, and finally 2 unique light/heavy pairings (LOB12.3-Lv4/Hv4 and LOB12.3-Lv4/Hv5) that were part of recombinant Fab fragments. Sequence numbering is according to Kabat et al.¹⁸ CDRs are shown in bold and were defined according to the sequence definition of Kabat et al.,¹⁸ except for CDR-H1, which is the combined sequence and structural definition.¹⁹