Figure 2.

Affinity maturation of 9A2 results in Fabs with improvements in dissociation rate (kd) and affinity (KD). Binding of (A) 9A2 Fab, (B) 9A2-VR24 Fab and (C) 9A2-V24.29/CSL311 Fab to immobilized βc was determined by surface plasmon resonance. Sensorgram (colored) and fitted curves (black) for each concentration are shown. The kinetic constants can be found in Tables S1 and S2.