Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Apr 15;8(4):706–717. doi: 10.1080/19420862.2016.1156828

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Merck Sharp & Dohme Corp.

PMC Copyright notice

Figure 4. — Analysis of mAb A glycan profiles by UMAG in aqueous solution. (A) Analysis of mAb A digested by trypsin for 5 min (top) and undigested mAb A (bottom) by RP-HPLC. Undigested mAb A was injected at a 1/6 amount of digested antibody. (B) Different rates of cleavage by trypsin under aqueous conditions near the glycosylation site. The majority of glycopeptides were missed cleavage peptides with an internal trypsin site. (C) Comparison of glycoprofiles analyzed by organic UMAG under organic conditions (blue) (n = 2) and aqueous UMAG under aqueous conditions (yellow) (n = 4).