Figure 6.

Antibody-dependent cell-meditated cytotoxicity mediated by XGFR*. (A) Induction of in vitro ADCC on H460M2 cells by glycoengineered affinity matured antibody XGFR*. A non-glycoengineered XGFR* antibody was included as control. (B) Induction of in vitro ADCC on TC-71 cells by glycoengineered bispecific antibody XGFR* and parental antibody control mixture R7072/GA201. Tumor cells were seeded in 96-well plates with various antibody concentrations and human PBMC were added at an effector to target ratio E:T of 25:1. Plates were incubated for 4 hours at 37°C, 5% CO2. Cytotoxicity was measured using the LDH Cytotoxicity Detection Kit or the xCelligence system. At least 2 independent data sets with comparable results were obtained. Data points surrounded by square were excluded for curve fitting.