Table 2.
Surface plasmon resonance analysis of R1507 affinity maturation. Kinetic rate constants ka and kd as well as affinity (KD) of affinity-matured Fab fragments were measured by SPR.
| Clones | Analyte | ka (1/Ms) | kd (1/s) | KD (M) |
|---|---|---|---|---|
| F13B5 | hu IGF-1R | 1.70E + 05 | 2.50E-04 | 1.47E-09 |
| L37F7 | hu IGF-1R | 2.32E + 05 | 2.37E-04 | 1.02E-09 |
| L39D7 | hu IGF-1R | 3.51E + 05 | 2.70E-04 | 7.69E-10 |
| L31D11 | hu IGF-1R | 5.71E + 05 | 1.53E-04 | 2.69E-10 |
| L31D7 | hu IGF-1R | 5.23E + 05 | 5.38E-04 | 1.03E-09 |
| R1507 | hu IGF-1R | 1.53E + 05 | 2.71E-03 | 1.8E-08 |
Abbreviations: ka, association rate constant; kd, dissociation rate constant; KD, equilibrium constant.