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Initial qualification of conjugation using the microscale conjugation platform. Each of 3 antibodies was conjugated at microscale (3 replicates each, 100 µg per replicate) with SMCC-DM1 and SPDB-DM4 at pH 8, and with SMCC-DM1 at pH 6. (A) Yield as determined by volume measurement and concentration from SE-UPLC. (B) % Monomer and (C) DAR, each determined by SE-UPLC. Each bar is an average + SD of 3 replicates.
