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Example titrations of different human IgG1s with SMCC-DM1 using antibody stocks of different concentrations. Each point is the average of 2 replicate purified conjugation reactions. Each reaction contained 100 µL of antibody stock at the indicated concentration in a 200 µL reaction volume. Antibodies: (A) mAb A2; (B) mAb A3; (C) mAb A4.
