Fig. 2.

Synthesis of PLA1 from Serratia sp. in a cell-free protein synthesis system. The plasmid pET21a Serr PLA1 was incubated in the reaction mixture for cell-free protein synthesis as described in “Methods” section. After incubation for 3 h, total and soluble amounts of cell-free synthesized PLA1 were quantified by measuring the TCA-insoluble radioactivity of the samples (a). Embedded image of a shows the results of SDS-PAGE and western blot analysis. PLA1 activity produced per mg of endogenous cellular protein in E. coli or cell-free protein synthesis system was compared in b