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. 2016 Jul 29;9:159. doi: 10.1186/s13068-016-0563-5

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Purification of PLA1 expressed in each system. Cell-free synthesized PLA1 from a 3 mL reaction was purified through a Ni-NTA column (a). Turbidity of the crude sesame oil was cleared after incubation with the purified PLA1 (30 µg PLA1 in 10 mL oil) for 12 h at 40 °C, indicating that the cell-free synthesized enzyme catalyzed degumming of crude oil (b)