Fig. 4.

Cell-free synthesis of PLA1 in varying reaction volumes. The reaction scale of cell-free PLA1 synthesis was increased sequentially from 150 µL to 3 mL to 500 mL. The total and soluble productivity and titer of functional PLA1 were not significantly affected by the reaction volume. The bars in the graph represent total (blank) and soluble (filled) amounts of cell-free synthesized protein. Circles represent PLA1 activity measured in the reaction mixture. Error bars represent the standard deviation from three independent experiments