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. 2016 Jul 29;11(7):e0160160. doi: 10.1371/journal.pone.0160160

Fig 3. The comparison of HCD- and CID-type fragmentation using the back-to-back strategy (HCD-IT vs. CID-IT).

Fig 3

(A-D) Number of spectra for +2, +3, +4 and ≥5 charged ions using different data interpretation methods. (E-H) Percentage of spectra for for +2, +3, +4 and ≥5 charged ions using different data interpretation methods. (I-L) Log2 (HCD/CID ratio of score) distributions for +2, +3 and +4 charged peptides. Xcorr for SEQUEST, ion score for Mascot, SVM score for Percolator related combinations were presented. One replicate of the back-to-back LC-MS analyses of HCD-IT versus CID-IT was randomly selected.