Fig 3. Lipid profiles of Agrobacterium tumefaciens wild type and cls mutant strains.

Cells were cultivated in LB medium grown until late exponential phase (OD ~ 4) Total lipids of A. tumefaciens wild type, Δcls1, Δcls2 and Δcls1/Δcls2 were isolated and analyzed by 2D-TLC. Loss of CL in the Δcls1/Δcls2 strain is indicated by a dashed circle and accumulation of CL is marked by arrows. Lipids were visualized by CuSO₄-treatement. Abbreviations: PE (phosphatidylethanolamine); MMPE (monomethyl-PE); OL1 (ornithine lipid 1); OL2 (ornithine lipid 2); PC (phosphatidylcholine); PG (phosphatidylglycerol); CL (cardiolipin).