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. 2016 Jul 29;11(7):e0160373. doi: 10.1371/journal.pone.0160373

Fig 4. Gas chromatography MS analysis of membrane lipids from different Agrobacterium tumefaciens strains.

Fig 4

Cultures were harvested in early exponential phase before lipids were isolated, separated and analyzed as previously described [17]. Mass spectrometry (MS) was used to quantify the lipid (A) and total fatty acid compositions (B) of A. tumefaciens wild type and cls single and double mutants via fatty acid methyl esters. (C) Fatty acid composition of cardiolipins in different A. tumefaciens strains. CL was separated from other lipids by 2D-TLC. MS was used to identify CL-specific fatty acids. Data represent single measurements except for Δcls1/Δcls2 in (A) with n = 2. Abbreviations: wt (wild type); PE (phosphatidylethanolamine); MMPE (monomethyl-PE); OL1 (ornithine lipid 1); OL2 (ornithine lipid 2); PC (phosphatidylcholine); PG (phosphatidylglycerol); CL (cardiolipin).