Fig 8. Phenotypic characterization of A. tumefaciens wild type and Δcls1/Δcls2.

(A) Growth of A. tumefaciens wild type and a cls1/cls2 double mutant. Strains were cultivated in LB or M9 minimal medium and cell density was monitored over two days. (B) Detection of the type IV secretion system components VirB8 and VirB9 in different A. tumefaciens strains. Cells were grown under non-virulence-inducing conditions (- vir) or virulence-inducing conditions (+ vir) in AB minimal medium. Total proteins were separated via SDS-PAGE and screened for the presence of VirB8 and VirB9 using Western blot analysis with specific antisera. A strain lacking PC (ΔPC) was used as a negative control. (C) Representative images of A. tumefaciens-induced tumorigenesis on potato discs. Discs were infected with 10⁸ cells and incubated for 21 days before images were taken.