Fig 5. The electrophoretic mobility shift assay (EMSA) of Siganus canaliculatus Δ4 Fad core promoter with its liver proteins.
The reaction was carried out in lane 1 (no proteins, 5’ biotin labeled probe), lane 2 (liver cytoplasmic proteins, 5’ biotin labeled free probe), lane 3 (liver cytoplasmic proteins, unlabeled competitor probe, 5’ biotin labeled free probe), lane 4 (liver nucleus proteins, 5’ biotin labeled probe), lane 5 (liver nucleus proteins, unlabeled competitor probe, 5’ biotin labeled probe). Band A is gel shift of DNA-protein complexes. Band B is the free probe.