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(A) SDS-PAGE analysis of purified BVMOAFL838. Lane M: molecular weight marker, Lane 1: E. coli cell-free extract expressing BVMOAFL838, Lane 2: Purified BVMOAFL838. (B) Absorbance spectra of BVMOAFL838 before and after treatment with 8 M urea. Extinction coefficient of free FAD at 450 nm: 11.3 mM-1.cm-1, Enzyme bound FAD at 436 nm: 12.7 mM-1.cm-1 and 11.6 mM-1.cm-1 at 450 nm.
