Table 2. Biotransformations of aliphatic ketones (10 mM) by purified BVMOAFL838 (2 μM) and kinetic parameters determined from initial velocities of NADPH oxidation.
Substrate | Conversion (%) | TOF (s-1) | Km (mM) | ||
---|---|---|---|---|---|
2h | 8h | 10 mM kobs | |||
1a | 2-octanone | >99 | >99 | 5.3 | 0.01 ± 0.003 |
2a | 3-octanone | >99 | >99 | 6.6 | 0.17 ± 0.03 |
3a | 2-decanone | 87 | >99 | 6.8 | <0.01 |
4a | 2-undecanone | 42 | 98 | 6.1 | <0.01 |
5a | 2-dodecanone | 21 | 99 | 5.3 | <0.01 |