. 2016 Jul 29;11(7):e0160186. doi: 10.1371/journal.pone.0160186

Table 3. Biotransformations of aryl ketones and aldehydes by E. coli whole cells expressing BVMO_AFL838 (WC) and purified BVMO_AFL838 (ENZ).

Kinetic parameters determined from initial velocities obtained from NADPH oxidation.

Substrate		Conversion (%)			TOF (s^-1)	K_m (mM)
Substrate		WC_2h	ENZ_2h	ENZ_8h	10 mM k_obs
6a	Acetophenone	19	4	13	0.05	10.0 ± 1.2
7a	4-Hydroxyacetophenone	84	>99	>99	1.4	0.8 ± 0.05
8a	Phenylacetone	72	n.c.	n.c.	0.28^a	2.4 ± 1.2^a
9a	4-Phenyl-2-butanone	64	>99	>99	2.4	<0.01^b
10a	4-(4-Hydroxyphenyl)-2-butanone	85	>99	>99	2.8	<0.01^b
11a	4-(4-Methoxyphenyl)-2-butanone	68	85	85	4.3	<0.01^b
12a	Benzaldehyde	62	n.c.	n.c.	0.03	6.5 ± 0.7
13a	4-Methoxybenzaldehyde	63	81	88	1.0	0.03 ± 0.002

^a Determined using 2 μM of BVMO

^b Substrate inhibition observed, K_i>>K_m

Table 3. Biotransformations of aryl ketones and aldehydes by E. coli whole cells expressing BVMOAFL838 (WC) and purified BVMOAFL838 (ENZ).