TABLE 3:
Characteristics of polypeptides altered in d1blic (RNAi-A) flagella.
| Protein | V5.5 genome ID | Amino acid residues (molecular weight, kDa) | Predicted domains | Homo sapiens best hit |
|---|---|---|---|---|
| Decreased | ||||
| RSP17 | Cre07.g340400 | 926 (98) | ND | ND (Volvox, 0.0) |
| WNK1 | Cre17.g700133 | 733 (77) | S/T kinase | AURKA (NP_003591) 2e-39, aa 238–520 |
| FAP24 | Cre02.g081050 | 529 (59) | TroA-d | ND (Volvox, 2e-149) |
| PKD2 | Cre17.g715300 | 1644 (183) | Polycystin cation channel | PKD2L1 (NP_057196) 3e-19, aa 1056–1418 |
| FAP5 | Cre16.g687966 | 1307 (133) | TPR repeats | NHPH3 (NP_694972) 1e-22, aa 1001–1283 |
| FAP208 | Cre11.g482001 | 1101 (114) | ANK repeats | ANK2 (NP_066187) 1e-48, aa 676–1079 |
| Predicted protein | Cre17.g747247 | 1846 (185) | AAA, TPR repeats | KLC1 (NP_005543) 6e-19, aa 1585–1781 |
| FAP26 | Cre04.g215250 | 2462 (partial) | ANK repeats | ND (Volvox, 2e-173) |
| FAP48/IP3R | Cre16.g665450 | 3210 (357) | Ins145_P3, RIH, RYDR_ITP, RYDR_IT | ITPR2 (NP_002214) 9e-30, aa 132–977 |
| Increased | ||||
| FAP256 | Cre10.g431150 | 862 (92) | TPR repeat | CEP104 (NP_055519) 1e-72, aa 7–672 |
| RB47 | Cre01.g039300 | 657 (70) | RRM and PolyA binding | PABPC1 (NP_002559) 0.0, aa 17–657 |
| Alanine tRNA synthetase | Cre02.g143200 | 1164 (122) | Alanyl tRNA synthetase, DHHA1 | Alanine tRNA ligase (NP_001596) 0.0, aa 223–1164) |
| HSP90A | Cre09.g386750 | 705 (81) | ATPase, HSP90 | HSP90α2 (NP_005339) (0.0, aa 4–705) |
| ABC4/EF-3 | Cre04.g222700 | 1053 (115) | ABC transporter, Chromo | ABCF3 (NP_060828) 2e-43, aa 450–788 |
| IPY3 | Cre09.g387875 | 192 (22) | Pyrophosphatase | PPA1 (NP_066952) 2e-11, aa 5–149 |
| GDP-d-mannose phosphorylase | Cre16.g672800 | 360 (40) | Nucleotidyl transferase | GMPPB (NP_068806) 3e-154, aa 1–360 |
| GST | Cre16.g682725 | 217 (24) | GST domains | GSTA1 (NP_665683) 2e-20, aa 4–203 |
| FAP295 | Cre16.g663200 | 707 (80) | PK, cNTP binding | PRKG2 (NP_006250) 1e-99, aa 27–663 |
| PRX2 | Cre02.g114600 | 198 (22) | Peroxiredoxin | PRDX4 (NP_006397) 1e-94, aa 3–198 |
| FAP179 | Cre17.g742300 | 219 (25) | GST domains | GSTP1 (NP_000843) 5e-29, aa 5–216 |
| ICL1 | Cre06.g282800 | 417 (46) | Isocitrate lyase | ND (Volvox, 0.0) |
| Unknown | Cre05.g239500 | 704 (73) | AAA | ND (Volvox, 2e-58) |
The predicted amino acid sequence in the Chlamydomonas genome V5.5 was blasted against the National Center for Biotechnology Information database to identify the most closely related sequence in H. sapiens. The name of the best hit, its accession number, blast score, and region of alignment are shown. If no significant hit was detected (ND), then the blast score for the Volvox orthologue is shown.