TABLE 3:
Strains used in this study.
| Name | Genotype | Source |
|---|---|---|
| BY4741a | MATa his3∆0 leu2∆0 met15∆0 ura3∆0 ho | Winzeler et al. (1999) |
| BY4742a | MATα his3∆0 leu2∆0 lys2∆0 ura3∆0 ho | Winzeler et al. (1999) |
| JHY14b,c,d | MATα lys2 ura3-52 leu2-3112 trp1-∆1 mmm1-∆1::LEU2 VPS13-G820D [ρ+, TRP1] | This study |
| JKY2b | MATα lys2 ura3-52 leu2-3112 trp1-∆1 vps13∆::KAN-MX4 [ρ+, TRP1] | This study |
| KWY70b | MATα lys2 ura3-52 leu2-3112 trp1-∆1 mmm1-∆1::LEU2 VPS13-G820R [ρ+, TRP1] | This study |
| KWY75b | MATα lys2 ura3-52 leu2-3112 trp1-∆1 mmm1-∆1::LEU2 VPS13-V1210E [ρ+, TRP1] | This study |
| KWY76b | MATα lys2 ura3-52 leu2-3112 trp1-∆1 mmm1-∆1::LEU2 VPS13-N1467H [ρ+, TRP1] | This study |
| KWY80b | MATα lys2 ura3-52 leu2-3112 trp1-∆1 mmm1-∆1::LEU2 VPS13-P268R [ρ+, TRP1] | This study |
| KWY105b | MATα lys2 ura3-52 leu2-3112 trp1-∆1 mmm1-∆1::LEU2 VPS13-G1245S [ρ+, TRP1] | This study |
| KWY109b | MATα lys2 ura3-52 leu2-3112 trp1-∆1 mmm1-∆1::LEU2 VPS13-G718K [ρ+, TRP1] | This study |
| KWY111b | MATα lys2 ura3-52 leu2-3112 trp1-∆1 mmm1-∆1::LEU2 VPS13-L984S [ρ+, TRP1] | This study |
| KWY114b | MATα lys2 ura3-52 leu2-3112 trp1-∆1 mmm1-∆1::LEU2 VPS13-A1512E [ρ+, TRP1] | This study |
| KWY131b | MATα lys2 ura3-52 leu2-3112 trp1-∆1 mmm1-∆1::LEU2 VPS13-G718D [ρ+, TRP1] | This study |
| MTY71b,c,d | MATα lys2 ura3-52 leu2-3112 trp1-∆1 VPS13-L822F [ρ+, TRP1] | This study |
| MTY79b,c,d | MATα lys2 ura3-52 leu2-3112 trp1-∆1 mmm1-∆1::LEU2 VPS13-L822F [ρ+, TRP1] | This study |
| PTY33b | MATa ade2 ura3-52 leu2-3112 trp1-∆1 [ρ+, TRP1] | Thorsness and Fox (1993) |
| PTY44b | MATα lys2 ura3-52 leu2-3112 trp1-∆1 [ρ+, TRP1] | Thorsness and Fox (1993) |
| PTY66b,d | MATα lys2 ura3-52 leu2-3112 trp1-∆1 vps13-31 [ρ+, TRP1] | Thorsness and Fox (1993) |
| THY23b | MATα lys2 ura3-52 leu2-3112 trp1-Δ1 mmm1-Δ1::LEU2 [ρo] | Hanekamp et al. (2002) |
| JSYD10 | MATα leu2 his3∆ MET15 ura3 arg4-NspI lys2 trp1::hisG ho | This study |
| MATa leu2∆0 his3∆0 met15∆0 ura3∆0 ARG4 LSY2 TRP1 ho | ||
| mmm1∆::kanMX6 VPS13 | ||
| MMM1 vps13-GFPc::HIS3MX6 | ||
| GCY1a | MATa his3∆0 leu2∆0 met15∆0 ura3∆0 ho vps13-GFPc::HIS3MX6 | Huh et al. (2003) |
| GCY2a | MATa his3∆0 leu2∆0 met15∆0 ura3∆0 ho DID2::GFPc::HIS3MX6 | Huh et al. (2003) |
| GCY3a | MATa his3∆0 leu2∆0 met15∆0 ura3∆0 ho NVJ1::GFP::HIS3MX6 | Huh et al. (2003) |
| GCY4a | MATa his3∆0 leu2∆0 met15∆0 ura3∆0 ho VAC8::GFP::HIS3MX6 | Huh et al. (2003) |
| KO1a | MATa his3∆0 leu2∆0 met15∆0 ura3∆ ho vps13∆::kanMX6 ho | Winzeler et al. (1999) |
| KO3a | MATa his3∆0 leu2∆0 met15∆0 ura3∆0 vps10∆::kanMX6 ho | Winzeler et al. (1999) |
| KO4a | MATa his3∆0 leu2∆0 met15∆0 ura3∆0 ho mmm1∆::kanMX6/pRS316-MMM1 | Winzeler et al. (1999) |
| KO5a | MATa his3∆0 leu2∆0 met15∆0 ura3∆0 ho atg32∆::kanMX6 | Winzeler et al. (1999) |
| JSP461-1a | MATa his3∆0 leu2∆ ura3∆ ho vps13∆::HIS3MX6 atg32∆::kanMX6 | This study |
| JSP497a | MATa his3∆0 leu2∆0 met15∆0 ura3∆0 ho VPS13-GFP1360 | This study |
| JSP513a | MATα his3∆0 leu2∆0 lys2∆0 ura3∆0 ho GFPN-vps13 | This study |
| RP201a | MATa his3∆0 leu2∆0 met15∆0 ura3∆0 ho vps13-L66P | This study |
| RP202a | MATa his3∆0 leu2∆0 met15∆0 ura3∆0 ho vps13-L1107P | This study |
| RP203a | MATa his3∆0 leu2∆0 met15∆0 ura3∆0 ho vps13-Y2702C ho | This study |
| RP205a | MATa his3∆0 leu2∆0 met15∆0 ura3∆0 ho vps13-C89K | This study |
| LUKE3a | MATa his3∆0 leu2∆0 met15∆0 ura3∆0 ho VPS13-GFP1360 mmm1∆::kanMX6 ho/pRS316-MMM1 | This study |
| JSP499-1a | MATα his3∆0 leu2∆0 lys2∆0 ura3∆0 ho VPS13-GFP1360 | This study |
| JSP527a | MATa leu2∆0 his3∆0 LYS2 met15∆0 ura3∆0 ho DID2::mRFP VPS13 | This study |
| MATα leu2∆0 his3∆0 lys2∆0 MET15 ura3∆0 ho DID2 VPS13::GFP1360 | ||
| JSP512a | MATa his3∆0 leu2∆0 lys2∆0 ura3∆0 ho VPS13-GFP1360 DID2::mRFP | This study |
| JSP541a | MATa his3∆0 leu2∆0 met15∆0 ura3∆0 ho VPS13-GFP1360 nvj1Δ::kanMX6 | This study |
| JSP545a | MATa his3∆0 leu2∆0 met15∆0 ura3∆0 ho VPS13-GFP1360 vac8∆::kanMX6 | This study |
| JSP547a | MATa his3∆0 leu2∆0 met15∆0 ura3∆0 ho NVJ1::GFP::HIS3MX6 vac8∆::kanMX6 | This study |
| JSP555a | MATa his3∆0 leu2∆0 met15∆0 ura3∆0 ho VAC8::GFP::HIS3MX6 nvj1∆::kanMX6 | This study |
| JSP495a | MATa his3∆0 leu2∆0 met15∆0 ura3∆0 ho DID2::mRFP | This study |
| JSP536a | MATa his3∆0 leu2∆0 met15∆0 ura3∆0 ho vps13∆::kanMX6 NVJ1::GFP::HIS3MX6 | This study |
| JSP531a | MATα his3∆0 leu2∆0 lys2∆0 ura3∆0 ho VPS13-G718K-GFP1360 | This study |
| JSP549a | MATα his3∆0 leu2∆0 lys2∆0 ura3∆0 ho VPS13-G718K | This study |
| JSP556a | MATα his3∆0 leu2∆0 lys2∆0 ura3∆0 ho vps13-G718K-GFPc::HIS3MX6 | This study |
| JSP560a | MATa his3∆1 leu2∆0 met 15∆0 ura3∆0 ho vps13-L66P-GFP1360 | This study |
| JSP563a | MATa his3∆1 leu2∆0 met 15∆0 ura3∆0 ho vps13-L66P G718K-GFP1360 | This study |
| JSP590 | MATa his3∆0 leu2∆0 ura3∆0 lys2∆0 ho VPS13-G718K-GFPc::HIS3MX6 mmm1∆::kanMX6/pRS316-MMM1 | This study |
| JSP577 | MATa his3 leu2 ura3 lys2 ho vps13-GFPc::HIS3MX6 mmm1∆::kanMX6 /pRS316-MMM1 | This study |
| JSP442 | MATα his3∆ arg4-NspI leu2 lys2 trp1::hisG ura3 mmm1∆::kanMX6 | This study |
| JSP443 | MATa leu2 trp1::hisG ura3 his3∆ ho vps13∆::HIS3MX6 mmm1∆::kanMX6 /pRS316-MMM1 | This study |
| JSP491 | MATα ura3 leu2 trp1::hisG his3∆ lys2 ho GFPN-vps13 mmm1∆::kanMX6 | This study |
| JSP552 | MATa his3 leu2 lys2 ura3 ho VPS13::GFP1360 PADH1::NVJ1::3xmCherry::hphNT1 | This study |
| JSP446 | MATα his3 leu2 trp1::hisG ura3 ho vps13∆::HIS3MX6 mmm1∆::kanMX6 PDIT1-lacZ::LEU2 /pRS316-MMM1 | This study |
| JSP582 | MATa his3 ura3 vps13-C89K mmm1∆::kanMX6/ pRS316-MMM1 | This study |
| JSP585 | MATα his3 leu2 ura3 trp1::hisG ho vps13-L1107P mmm1∆::kanMX6 /pRS316-MMM1 | This study |
| RP301e | MATa ura3 leu2 trp1-hisG his3∆ lys2 ho∆::LYS2 VPS13-L66P mmm1∆::kanMX6/ pRS316-MMM1 | This study |
| JSP533e | MATa ura3 leu2 trp1-hisG his3∆ lys2 ho::LYS2 vps13-L66P G718K mmm1∆::kanMX6/pRS316-MMM1 | This study |
| JSP417e | MATα his3∆SK arg4-NspI leu2 ura3 trp1::hisG ho vps13∆::HIS3MX6 | This study |
| JSP441 | MATα his3∆ leu2 trp1::hisG ura3 ho mmm1∆::kanMX6 /pRS316-MMM1 | This study |
| JSP490e | MATa ura3 leu2 trp1::hisG his3∆ lys2 ho::LYS2 GFPN-vps13 | This study |
| CUY9002 | MATα leu2-3112 ura3-52 his3-Δ200 trp1-∆101 lys2-801 suc2-∆9 GAL natMX4-PTEF1-GFP-VPS39 PADH1-NVJ1-3xmCherry-hphNT1 | Honscher et al. (2014) |
| HI27e | MATα ura3 his3∆SK trp1::hisG arg4-NspI lys2 ho∆::LYS2 rme1::LEU2 leu2 vps13∆::HIS3MX6 ho | Nakanishi et al. (2007) |
| HI28e | MATa ura3 his3∆SK trp1::hisG lys2 ho∆::LYS2 leu2 vps13∆::HIS3MX6 | Nakanishi et al. (2007) |
| AN117-16De | MATa ura3 leu2 trp1::hisG his3∆SK lys2 ho∆::LYS2 | Neiman et al. (2000) |
| JSP528e | MATa leu2 VPS13-GFP1360 his3∆SK arg4-NspI ura3 trp1::hisG lys2 ho:: LYS2 | This study |
| MATα LEU2 VPS13-GFP1360 his3∆SK ARG4 ura3 trp1::hisG lys2 ho:: LYS2 | ||
| JSYD1f | MATa leu2∆0 his3∆0 met15∆0 ura3∆0 TRP1 ARG4 LYS2 ho | This study |
| MATα leu2 his3∆SK MET15 ura3 trp1::hisG arg4-NspI lys2 ho∆::LYS2 | ||
| RME1 VPS13 | ||
| rme1::LEU2 VPS13 | ||
| JSYD2f | Same as JSYD1 only vps13∆::kanMX6 | This study |
| vps13∆::HIS3MX6 | ||
| JSYD3f | Same as JSYD1 only vps13-GFPc::HIS3MX6 | This study |
| vps13∆::HIS3MX6 | ||
| JSYD4f | Same as JSYD1 only VPS13-GFP1360 | This study |
| vps13∆::HIS3MX6 | ||
| JSYD5f | Same as JSYD1 only GFPN-vps13 | This study |
| vps13∆::HIS3MX6 | ||
| JSYD6f | Same as JSYD1 only vps13-L66P | This study |
| vps13∆::HIS3MX6 | ||
| JSYD7f | Same as JSYD1 only vps13-C89K | This study |
| vps13∆::HIS3MX6 | ||
| JSYD8f | Same as JSYD1 only vps13-L1107P | This study |
| vps13∆::HIS3MX6 | ||
| JSYD9f | Same as JSYD1 only vps13-Y2702C | This study |
| vps13∆::HIS3MX6 |
aIsogenic with the BY4741 strain background.
bStrains are isogenic and were derived from D273-10B (Sherman, 1963).
cThe mitochondrial genotype is bracketed.
dThe vps13-31 allele was previously designated yme3-1 (Thorsness and Fox, 1993). The VPS13-G820D allele was previously designated YNT61-1 (Hanekamp et al., 2002). The VPS13-L822F allele had four previous designations; YNT61-2, YNT61-3, YNT61-4, and YNT61-5 (Hanekamp et al., 2002).
eIsogenic with the SK1 background.
fStrains are hybrids derived by crossing haploids from the BY4741 and SK1 background.