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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Methods. 2016 Mar 21;105:109–118. doi: 10.1016/j.ymeth.2016.03.009

Figure 1. Single-molecule fluorescence assays for monitoring the diffusion of dsRBPs on dsRNA.

Figure 1

A–C) Simplistic schemes to show the working principles of 1-color, 2-color and 3-color single-molecule fluorescence assays for studying the diffusion of dsRBPs on dsRNA. D) 1-color fluorescence assay using smPIFE shows the fluctuation of Cy3 intensity induced by the diffusion motion of TRBP on dsRNA. E) 2-color fluorescence assay using smFRET shows the distance change between TRBP and dsRNA due to the diffusion motion of TRBP. F) 3-color fluorescence assay using 3-color smFRET shows the anti-correlated intensity change between two acceptors located at either end of dsRNA due to the diffusion of TRBP on dsRNA.