Skip to main content
. Author manuscript; available in PMC: 2017 Jul 28.
Published in final edited form as: Cell. 2016 Jun 30;166(3):679–690. doi: 10.1016/j.cell.2016.05.070

Figure 3. Codon Pair-Mediated Inhibition Affects Translation and is Suppressed by Particular tRNAs.

Figure 3

(A) Wobble decoding is prevalent in the 17 inhibitory codon pairs.

(B) Flow cytometry scatter plots show GFPFLOW from two sets of variants that contain an inhibitory pair (top panels) or a synonymous optimized pair (bottom panels), in cells with either an empty vector or a plasmid expressing the indicated tRNA; the non-native exact matching tRNA is also indicated by a star.

(C) The effect on the GFPFLOW ratio of expressing a tRNA that decodes the 3′ codon in an inhibitory pair. Vector (blue), native tRNA (light purple), non-native tRNA (dark purple). Error bars represent SD. NA, not applicable; ND, not determined.