(A) mRNA expression levels of CXCL1, CXCL2, IL-6, TNF-α, and IL-1β in neutrophils following incubation with C. neoformans in the presence of mouse plasma. (B) Comparisons of mRNA expression levels of chemokines and proinflammatory cytokines in neutrophils 30 min after incubation with C. neoformans in the presence of wild-type or C3−/− mouse plasma. (C) Secretion of CXCL1 in the lung (homogenate of the lung tissues in 2 ml PBS) of wild-type or C3−/− mice (n=5) following intravenous injection with C. neoformans. Mice instilled intranasally with LPS were used as positive control. Data are presented as means ± SEM. Data are representative of results from 2 independent experiments. *, p<0.05; **, p<0.01.