Figure 5. Organ-specific progenitor cell dominance.

(A) The indicated organs were dissected from a single adult v7 transgenic edited zebrafish (ADR1). A blood sample was collected as described in the Methods. The heart was further split into the four samples shown (fig. S10). (B) Patterns of editing in the most prevalent 25 alleles (out of 135 total) recovered from the blood sample. Layout as described in the Fig. 1B legend. The most prevalent 5 alleles (indicated by asterisks) comprise >98% of observed cells. (C) Patterns of editing in the most prevalent 25 alleles (out of 399 total) recovered from brain. Layout as described in the Fig. 1B legend. Alleles that have identical editing patterns compared to the most prevalent blood alleles are indicated by asterisks and light shading. (D) The five dominant blood alleles (shades of red) are present in varying proportions (10–40%) in all intact organs except the FACS-sorted cardiomyocyte population (0.5%). All other alleles are summed in grey. (E) The cumulative proportion of cells (y-axis) represented by the most frequent alleles (x-axis) for each adult organ of ADR1 is shown, as well as the adult organs in aggregate. In all adult organs except blood, the five dominant blood alleles are excluded. All organs exhibit dominance of sampled cells by a small number of progenitors, with fewer than 7 alleles comprising the majority of cells. For comparison, a similar plot for the median embryo (dashed) from each time-point of the developmental time course experiment is also shown. (F) The distribution of the most prevalent alleles for each organ, after removal of the five dominant blood alleles, across all organs. The most prevalent alleles were defined as being at >5% abundance in a given organ (median 5 alleles, range 4–7). Organ proportions were normalized by column and colored as shown in legend. Underlying data presented in table S2.