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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Methods. 2016 Apr 27;105:119–127. doi: 10.1016/j.ymeth.2016.04.030

Figure 5.

Figure 5

RecQ unwinding measurements under different conditions and DNA geometries. (a) Mean unwinding rate plotted as a function of Na+ concentration and compared to the calculated unwinding rate based on models in which the rate is determined solely by the DNA base-pair energy. (b) Mean unwinding rate plotted as a function of ATP concentration and fit with the Hill equation (see main text). (c) The mean unwinding rate plotted as a function of temperature and compared to the predicted rate as a function of temperature: ν(T) = ν(21°C)T/T(21°C). (d) RecQwt shows dramatically different unwinding behavior with two different DNA geometries. RecQwt slowly and inefficiently unwinds hairpin DNA due to long and frequent pauses, whereas it efficiently and rapidly unwinds gapped DNA.