Table 2.
Optical microscopical biophysical techniques applied in oligodendrocyte-myelin field in chronological order
| References | Biophysical techniques | Major findings |
|---|---|---|
| [182] | FCS | Diffusion coefficient of MOG-eGFP and Bodipy FL-C5 sphingomyelin |
| [112] | C-Laurdan |
In co-culture systems (neuron-derived signals): Increased membrane order in primary OLGs by neuron-derived soluble factors Increased membrane condensation in the presence of MBP |
| FRAP | Formation of highly dynamic GalC clusters in OLGs | |
| STED | Increased number of large GalC clusters in the presence of neurons | |
| FRET | Self-interaction of GalC within the large clusters | |
| [136] | FRET | Decrease in Rho activity in Oli-neu cells in the presence of neuronal-conditioned medium |
| C-Laurdan | Increased membrane condensation in the presence of conditioned neuronal medium or RhoGTPase inactivation | |
| [183] | RICS, FRAP | Diffusion coefficient of MOG-eGFP in OLN-93 cells |
| [164] | FRET | Interaction of 14-kDa MBP with PIP2 (sensed by CFP-PH-PLCδ1) in Oli-neu cells |
| [184] | RICS, FRAP | Diffusion coefficient of DiI-C18 in primary OLGs |
| [99] | C-Laurdan |
Higher lipid order in GUVs prepared from myelin compared to Oli-neu cells Higher lipid order in GUVs prepared from wild type animal compared to shiverer mousea or CerS2-deficient mouseb Higher lipid order in primary OLGs compared to FB1c-treated OLGs |
| FCS | Slower diffusion of DiD in GUVs prepared from wild type animal compared shiverera mouse or CerS2 deficient mouse | |
| FRAP | Slower diffusion of Cell MaskOranged in control OLGs compared to FB1c-treated OLGs | |
| [88] | FRAP |
Detection of highly mobile MAG in OLGs isolated from shiverer mouse compared to control to show the diffusion barrier function of 14-kDa MBP Higher mobility of truncated Tmem10 (without cytosolic domain) compared to full length Tmem10 |
| [87] | FRAP | Dynamic role of 21.5-kDa MBP-RFP in OLN-93 cells proliferation |
| [95] | FRAP | Decreased 14-kDa MBP diffusion due to its oligomerization |
| FRET | Self association of 14-kDa MBP | |
| [97] | s-FCS | Decreased mobility of PLP-eGFP in the presence of sulfatide (rather than GalC) on poly-l-lysine and laminin-2 in OLN-93 cells |
| Increased mobility of PLP-eGFP even in the presence of sulfatide on fibronectin in OLN-93 cells | ||
| z-scan FCS, z-scan RICS | Increased mobility of 18.5-MBP-eGFP in the presence of only GalC in OLN-93 cells | |
| [166] | FRET | Presence of one or more intermediate conformational states of 18.5-kDa MBP |
aMBP-deficient mouse
bMouse model unable to synthesize long acid fatty acyl chain lipids
cInhibitor of sphingolipid synthesis
dMembrane dye