Fig. 6.

In situ mutation of UBP1 in T. b. brucei. a Alignment of the predicted RNA recognition motif (RRM) of UBP1 from STIB900, STIB900-M, and STIB900-P alongside Trypanosoma cruzi UBP1. The mutation of the conserved Arg¹³¹ to Leu is highlighted in the alignment and Arg¹³¹ is indicated with an arrow in the published structure of TcUBP1. The full length TcUBP1 is 224 aa but a truncated version (1–139) including the RRM (35–126) was used to determine the structure [54]. Swiss model [61] was used to visualize the structure. b Sanger sequencing electropherograms of UBP1 amplified from genomic DNA of parental 2T1 cells and two transgenic clones H and L, showing the nucleotides that encode amino acids 130–132. c In vitro drug sensitivities (IC₅₀ ± standard deviation; n = 12) for melarsoprol and pentamidine determined with the Alamar blue assay for parental 2T1 cells and the two transgenic clones H and L. There was no significant difference in drug sensitivity between the three lines (Anova one-way analysis of variance followed by Tukey’s multiple comparison test)