Table 1.
Peptide/Protein Target | Antigen Sequence (if Known) | Name of Antibody | Manufacturer, Catalog Number, and/or Name of Individual Providing the Antibody | Species Raised in; Monoclonal or Polyclonal | Dilution Used (Application) |
---|---|---|---|---|---|
S6K | N/A | p70 S6K (49D7) | Cell Signaling, 2708 | Rabbit; monoclonal | 1:1000 (WB) |
Phospho-S6K (T389) | N/A | Antiphospho-p70 S6K (Thr389) | Millipore, 04-392 | Rabbit; monoclonal | 1:1000 (WB) |
Phospho-S6 (S240/244) | N/A | Phospho-S6 ribosomal protein (Ser240/244) (D68F8) | Cell Signaling, 5364 | Rabbit; monoclonal | 1:1000 (IHC) |
c-Fos | N/A | c-Fos antibody (K-25) | Santa Cruz Biotechnology, Inc, sc-253 | Rabbit; polyclonal | 1:2000 (IHC) |
Rabbit IgG | N/A | Antirabbit IgG, HRP-linked antibody | Cell Signaling, 7074 | Goat | 1:5000 (WB) |
Rabbit IgG | N/A | Biotinylated goat antirabbit IgG antibody | Vector, BA-1000 | Goat | 1:200 (IHC) |