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. 2016 Jun 1;157(8):3096–3107. doi: 10.1210/en.2015-2042

Figure 3.

Figure 3.

Knockdown of Bmal1 inhibited the matrix proteoglycan synthesis in chondrocytes, which was further aggravated by IL-1β. A, The proliferation of chondrocytes was determined using the CCK-8 assay after transfection with Bmal1 siRNA. B and C, Representative images of alcian blue staining and the levels of alcian blue-stained proteoglycans were detected by OD values at 620 nm. D, The chondrocytes were treated with IL-1β for 24 hour after the transfection of Bmal1 siRNA. Quantitative results for col2a1 and aggrecan in chondrocytes were measured by real-time PCR after transfection with Bmal1 siRNA under stimulation with IL-1β (10 ng/mL). E, The expression of MMP1, MMP3, MMP13, and ADAMT5 in chondrocytes was measured by real-time PCR after transfection with Bmal1 siRNA under stimulation of IL-1β (10 ng/mL). F, The representative Western blots of the col2a1 and aggrecan, MMP 13, and ADAMTS5 after transfection with Bmal1 siRNA under stimulation of IL-1β. Bars represent a mean and SE of n = 5/group. control, control siRNA; siBmal1, Bmal1siRNA.