Figure 4.

Overexpression of Bmal1 neutralized the metabolic imbalance of chondrocytes induced by IL-1β. The chondrocytes were treated with IL-1β for 24 hours after transfection with the Bmal1 plasmid. A and B, The representative images of the chondrocyte transfection with the Bmal1 plasmid, and the transfection efficiency was evaluated by real-time PCR and Western blot. C, The quantitative results for col2a1 and aggrecan in chondrocytes transfected with Bmal1 under stimulation with IL-1β was determined by real-time PCR. D, Quantitative results for the expression levels of MMP13 and ADAMTS5 were measured by real-time PCR. Bars represent a mean and SE of n = 5/group. control, control plasmid; plaBmal1, Bmal1 plasmid.