Table 1.
Antibody Table
| Peptide/Protein Target | Antigen Sequence (if Known) | Name of Antibody | Manufacturer, Catalog Number, and/or Name of Individual Providing the Antibody | Species Raised (Monoclonal or Polyclonal) | Dilution Used |
|---|---|---|---|---|---|
| Bmal1 | BMAL1 antibody (H-170) | Santa Cruz Biotechnology, sc-48790 | Rabbit; polyclonal | 1:200 | |
| Sirt1 | SirT1 (D1D7) | Cell Signaling Technology, number 9475 | Rabbit; mAb | 1:1000 | |
| Anti-SIRT1 antibody | Abcam, ab110304 | Mouse; mAb | 1:200 | ||
| Rev-erbα | Rev-Erbα (E1Y6D) | Cell Signaling Technology, number 13418 | Rabbit; mAb | 1:1000 | |
| GAPDH | Anti-GAPDH antibody | Abcam, ab181602 | Rabbit; monoclonal | 1:10 000 | |
| per2 | Anti-PER2 antibody | Abcam, ab180655 | Rabbit; polyclonal | 1:100 | |
| NAMPT | Antivisfatin antibody | Abcam, ab45890 | Rabbit polyclonal | 1:1000 | |
| col2a1 | Anticollagen II antibody | Abcam, ab34712 | Rabbit; polyclonal | 1:5000 | |
| aggrecan | Antiaggrecan antibody | Abcam, ab36861 | Rabbit; polyclonal | 1:1000 | |
| MMP13 | Anti-MMP13 antibody | Abcam, ab39012 | Rabbit; polyclonal | 1:3000 | |
| ADAMTS5 | Anti-ADAMTS5 antibody | Abcam, ab41037 | Rabbit; polyclonal | 1:250 | |
| Rabbit IgG H&L | Antirabbit IgG H&L (Alexa Fluor 488) | Abcam, ab150077 | Goat; polyclonal | 1:200 |
Abbreviations: H&L, heavy and light chains; mAb, monoclonal antibody.