Table 2.
Frequencies of Calcium Oscillations in GnRH Neurons
| Paradigms | Period 1 (Peaks/min) | Period 2 (Peaks/min) | Period 3 (Peaks/min) | Period 4 (Peaks/min) | Cells (n) | Explants (N) | |
|---|---|---|---|---|---|---|---|
| a | SFM–Gal1–16 (100nM)–SFM | 2.33 ± 0.12 | 1.27 ± 0.14a | 1.74 ± 0.15a | n/a | 77 | 3 |
| b | SFM–Gal1–16 (30nM)–SFM | 2.24 ± 0.12 | 0.74 ± 0.09a | 1.76 ± 0.12a | n/a | 72 | 3 |
| c | SFM–Gal1–16 (10nM)–SFM | 2.07 ± 0.12 | 1.09 ± 0.10a | 1.63 ± 0.13a | n/a | 71 | 4 |
| d | SFM–galanin–SFM | 2.54 ± 0.08 | 1.19 ± 0.11a | 2.13 ± 0.11a | n/a | 114 | 3 |
| e | SFM–AAB–AAB + Gal1–16–SFM | 1.61 ± 0.16 | 0.80 ± 0.13a | 0.51 ± 0.10a | 1.64 ± 0.14 | 38 | 3 |
| f | SFM–AAB + PRG–AAB + PRG + Gal1–16–SFM | 1.87 ± 0.10 | 0.93 ± 0.10a | 0.64 ± 0.09a | 1.68 ± 0.11 | 68 | 3 |
| g | SFM–M617–SFM | 1.91 ± 0.14 | 0.90 ± 0.12a | 1.74 ± 0.13a | n/a | 52 | 3 |
| h | SFM–M1145–SFM | 1.81 ± 0.11 | 1.57 ± 0.11a | 1.93 ± 1.12a | n/a | 65 | 3 |
| i | SFM–Gal2–11–SFM | 1.67 ± 0.12 | 1.78 ± 0.09 | 1.80 ± 0.10 | n/a | 79 | 3 |
| j | SFM–AAB–AAB + M617–SFM | 1.92 ± 0.12 | 1.05 ± 0.11a | 0.40 ± 0.08a | 1.28 ± 0.12 | 64 | 3 |
| k | PTX pretreated (2–3 h): SFM–Gal1–16–SFM | 1.77 ± 0.10 | 1.83 ± 0.11 | 1.68 ± 0.10 | n/a | 82 | 3 |
| l | SFM–FSK–FSK + Gal1–16–SFM | 1.81 ± 0.10 | 2.37 ± 0.08a | 1.37 ± 0.11a | 1.77 ± 0.10 | 100 | 3 |
| m | SFM–IBMX–IBMX + Gal1–16–SFM | 1.91 ± 0.08 | 2.16 ± 0.07 | 1.05 ± 0.07a | 1.49 ± 0.08 | 168 | 3 |
| n | TPNQ pretreated (50 min): TPNQ–TPNQ + Gal1–16 | 2.27 ± 0.11 | 1.36 ± 0.12a | n/a | n/a | 79 | 3 |
| o | Cs pretreated (20 min): Cs–Cs + Gal1–16 | 1.25 ± 0.06 | 1.05 ± 0.05a | n/a | n/a | 71 | 3 |
| p | Ba pretreated (10 min): Ba–Ba + Gal1–16 | 0.95 ± 0.07 | 0.71 ± 0.05a | n/a | n/a | 80 | 3 |
| cc | SFM–Gal1–16–SFM | 2.39 ± 0.13 | 0.47 ± 0.07a | 1.02 ± 0.15a | n/a | 67 | 4 |
| gg | SFM–M617–SFM | 1.95 ± 0.16 | 0.59 ± 0.11a | 1.38 ± 0.20a | n/a | 39 | 4 |
Galanin rat (10nM), Gal1–16 (10nM), M617 (10nM), M1145 (10nM), Gal2–11 (10nM), AAB (20μM BIC, 10μM CNQX, and 10μM AP5), PRG (100nM), PTX (250ng/mL), FSK (1μM), IBMX (100μM), Cs (5mM), Ba (400μM), and TPNQ (100nM). Data are expressed in mean ± SEM, n cells, N explants. Rows above the thick line contain data from 7-day in vitro explants; rows below the thick line contain data from 14-day in vitro explants. Rows cc and gg are repetitions at 14 days in vitro of rows c and g, respectively.
a
Significant difference compared with the previous period (per 2 with per 1) or (per 3 with per 2) (Student's t test; P < .05).