Table 3.
Frequencies of Calcium Oscillations in GnRH Neurons
| Paradigms | Period 1 (Peaks/min) | Period 2 (Peaks/min) | Period 3 (Peaks/min) | Period 4 (Peaks/min) | Cells (n) | Explants (N) | |
|---|---|---|---|---|---|---|---|
| a | SFM–AAB + PRG–AAB + PRG + M15–SFM | 2.35 ± 0.10 | 1.52 ± 0.12a | 0.63 ± 0.08a | 1.53 ± 0.12 | 101 | 3 |
| b | SFM–M15–M15 + Gal1–16–SFM | 1.90 ± 0.09 | 0.67 ± 0.10a | 0.55 ± 0.11a | 1.33 ± 0.11 | 61 | 3 |
| c | SFM–Gal1–16–Gal1–16 + M15–SFM | 2.28 ± 0.13 | 1.36 ± 0.15a | 0.75 ± 0.13a | 1.32 ± 0.15 | 52 | 3 |
| d | PTX pretreated (2–3 h): SFM–M15–SFM | 1.91 ± 0.09 | 1.49 ± 0.08a | 1.78 ± 0.09a | n/a | 134 | 4 |
| e | Cs pretreated (20 min): Cs–Cs + M15 | 0.77 ± 0.05 | 0.85 ± 0.06 | n/a | n/a | 57 | 3 |
Gal1–16 (10nM), AAB (20μM BIC, 10μM CNQX, and 10μM AP5), PRG (100nM), PTX (250ng/mL), Cs (5mM), and M15 (10nM). Data are expressed in mean ± SEM, n cells, N explants.
a
Significant difference compared with the previous period (per 2 with per 1) or (per 3 with per 2) (Student's t test; P < .05).