Table 4.
Effect of E2 on Trabecular Bone Mass in POMC-ERα−/− Mice
| Control |
POMC-ERα−/− |
|||
|---|---|---|---|---|
| OVX | OVX + E2 | OVX | OVX + E2 | |
| Femur | ||||
| BV/TV (%) | 9.5 ± 1.0 | 39.8 ± 1.9c | 9.2 ± 1.0 | 47.4 ± 2.0c,d |
| Tb.N (L/mm) | 2.4 ± 0.2 | 9.6 ± 0.4c | 2.3 ± 0.2 | 10.6 ± 0.6c |
| Tb.Th (μm) | 39 ± 1 | 42 ± 1 | 40 ± 1 | 45 ± 2 |
| Tb.Sp (μm) | 132 ± 1 | 74 ± 5c | 132 ± 1 | 60 ± 5c |
| Vertebrae L5 | ||||
| BV/TV (%) | 15.7 ± 1.1 | 26.8 ± 1.6c | 16.4 ± 1.7 | 34.2 ± 2.8b |
| Tb.N (L/mm) | 4.1 ± 0.3 | 6.3 ± 0.4b | 3.9 ± 0.5 | 8.1 ± 1.0a |
| Tb.Th (μm) | 39 ± 1 | 43 ± 1a | 42 ± 1 | 43 ± 3 |
| Tb.Sp (μm) | 71 ± 3 | 59 ± 2a | 74 ± 5 | 50 ± 4b |
High-resolution μCT analysis of BV/TV, trabecular number (Tb. N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp) in femur (n = 7–10) and vertebrae L5 (n = 4–6) of OVX POMC-ERα−/− and OVX control mice treated with either E2 (0.5 μg/d) or vehicle. Data are presented as mean ± SEM.
a
P < .05 E2 treated vs vehicle treated, Student's t test.
b
P < .01 E2 treated vs vehicle treated, Student's t test.
c
P < .001 E2 treated vs vehicle treated, Student's t test.
d
P < .05 E2 effect in POMC-ERα−/− mice vs E2 effect in control mice (interaction term from two-way ANOVA).