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. 2016 Jun 2;157(8):3278–3292. doi: 10.1210/en.2015-2046

Figure 4.

Figure 4.

Loss of function of TRβ increases Runx2 and Runx2 target genes. A, Western blot analysis illustrates that siRNA (TRβ) transfection into normal thyroid cells (ORI) results in a concentration-dependent decrease in TRβ protein and increase in Runx2 protein compared with scrambled nonspecific siRNA (ns) (upper panel). Graph summarizes quantitation, mean ± SD, of 3 separate experiments. Protein levels were standardized to β-actin and compared with respective controls. Significances at P < .01 are indicated (+). B, qRT-PCR illustrates that siRNA (TRβ) transfection results in corresponding changes in mRNA. Graph summarizes quantitation, mean ± SD of 3 separate experiments, of TRβ and Runx2 mRNA levels standardized to β-actin. Significance at P ≤ .01 compared with respective control is indicated (+). C, Knockdown of TRβ impacts Runx2 target genes; MMP13, MMP2, cyclin D1, osteopontin (OPN), and cadherin 6. qRT-PCR levels of target genes were standardized to β-actin. Data are the averaged fold change in transfected cells normalized to mock-transfected cells. Graph summarizes quantitation, mean ± SD of 3 separate experiments. Significances at P ≤ .01 compared with respective control are indicated (+).