Table 1.
Antibody Table
| Peptide/Protein Target | Antigen Sequence (if Known) | Name of Antibody | Manufacturer, Catalog Number | Species Raised in; Monoclonal or Polyclonal | Dilution Used | Technique |
|---|---|---|---|---|---|---|
| TRβ-1 | Human TRβ-1 | TRβ-1 antibody (J52) | Thermo Scientific, MA1-216 | Mouse; monoclonal | 1:500 | WB |
| TRβ | Synthetic peptide derived from human TRβ | Anti-TRβ antibody | Abcam, AB53170 | Rabbit; polyclonal | 1:500 | WB |
| Runx2 | Amino acids 294–363 of Runx2 of mouse origin | RUNX2 (M70) antibody | Santa Cruz Biotechnology, Inc, sc-10758 | Rabbit; polyclonal | 1:750 | WB |
| β-Actin | Affinity-purified anti-β-actin | Rockland Immunochemicals, 600-401-886 | Rabbit; monoclonal | 1:20 000 | WB | |
| TRβ | GMSEACLHRKSHSERRSTLK | Anti-TRβ antibody | Abcam, AB15545 | Rabbit; polyclonal | 1:500 | IF |
| Runx2 | RUNX2 partial recombinant protein with GST tag | Anti-RUNX2 (251–350) mAb | Abnova, H00000860-M06 | Mouse; monoclonal | 1:500 (IF), 1:100 (IHC) | IF, IHC |
| TRβ | Synthetic peptide corresponding to amino acids 31–50 of human THRB | Anti-THRB pAb | Abnova, PAB11824 | Rabbit; polyclonal | 1:100 | IHC |
| Runx1 | Synthetic peptide corresponding to amino acids near the amino terminus of human AML1 | AML1 antibody | Cell Signaling, CS4334 | Rabbit; polyclonal | 1:100 | IHC |
| TRβ | Recombinant fragment corresponding to a region within amino acids 1 and 199 of human TRβ | TRβ antibody | Thermo Scientific, PA5-29684 | Rabbit; polyclonal | ChIP | |
| Runx2 | KLH-conjugated synthetic peptide between 445–474 amino acids surrounding S533 of human RUNX2 | RUNX2 antibody | Thermo Scientific, PA5-14-816 | Rabbit; polyclonal | ChIP | |
| Rabbit-γ globulin | Pierce, 31887 | Rabbit; polyclonal | ChIP | |||
| TRβ | Purified fragment of human TRβ-1 corresponding to residues 201–456 | TR antibody (C3) | Thermo Scientific, MA1-215 | Mouse; monoclonal | EMSA | |
| Goat antimouse HRP | Goat antimouse IgG, H&L chain specific, peroxidase conjugate | Calbiochem EMD, 401253 | Goat; polyclonal | 1:5000 | WB | |
| Mouse antirabbit HRP | Peroxidase-conjugated IgG fraction monoclonal mouse antirabbit IgG; light chain specific | Jackson ImmunoResearch, 211-032-171 | Mouse; polyclonal | 1:10 000 | WB | |
| Goat antirabbit | Goat antirabbit Alexa Fluor 555 | Invitrogen/Life Technologies, A21429 | Goat; polyclonal | 1:500 (IF), 1:1000 (IHC) | IF, IHC | |
| Goat antirabbit | Goat antirabbit Alexa Fluor 488 | Invitrogen/Life Technologies, A11001 | Goat; polyclonal | 1:500 (IF), 1:1000 (IHC) | IF, IHC |