Effect of high-fat diet on body weight, fat mass, and lean mass in untreated wild-type and BChE-KO mice, as well as KO mice given AAV-luciferase or BChE vector. A, Body weights of male control (n = 27), BChE KO (n = 28), and BChE KO mice transduced with AAV encoding luciferase (LUC) (n = 10) or mouse BChE (n = 9). All mice were fed on 45% calorie high-fat diet. Two-way ANOVA showed significant differences in body weight: BChE KO VS wild type (P < .001), BChE KO VS LUC-vector-treated KO (not significant, n.s.); BChE KO VS BChE-vector-treated KO (P < .001), BChE-vector-treated KO VS wild type (n.s.). B, Fat and lean mass measured by Eco-MRI every 2 months. Results are mean ± SEM; **, BChE KO and LUC-vector treated-BChE KO vs wild-type group, P < .01. There were no significant group differences in lean mass.