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Energy expenditure and spontaneous activity across light and dark cycles. VO2 (A) and CO2 production (B), respiratory exchange ratio (C), spontaneous locomotor activity (D), and energy expenditure (E) were measured in Comprehensive Lab Animal Monitoring System metabolic chambers. All mice were 7 months old and had been on HFD for 6 months. Data are mean ± SEM (n = 8); **, P < .01 vs wild-type control.
