Fig. 12.

PI3-kinase inhibitor LY294002 does not affect PDGF-induced calpain activation and calpain-2-induced Akt phosphorylation. A: PASMCs were incubated with PDGF-BB (10 ng/ml) in the presence and absence of LY294002 (50 μM) for 30 min, after which calpain activity was measured, as described in materials and methods. B and C: PASMCs were transfected with plasmids containing human calpain-2 gene or empty plasmids and then incubated with and without LY294002 (50 μM) for 24 h, after which P-Akt-S473, P-Akt-T308, and total Akt were measured by Western blot. B: representative immunoblots of P-Akt-S473, P-Akt-T308, and total Akt. C: bar graph showing the changes in protein levels of P-Akt-S473 and P-Akt-T308. Values are means ± SE; n = 3. *P < 0.05 vs. control in vehicle. **P < 0.05 vs. empty vector in vehicle. #P < 0.05 vs. empty vector in LY294002.