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. 2016 Apr 20;311(1):C24–C34. doi: 10.1152/ajpcell.00295.2015

Fig. 4.

Fig. 4.

Overexpression of calpain-2 increases the levels of P-Akt-S473 and P-Akt-T308. PASMCs were transfected with plasmids containing human calpain-2 gene or empty plasmids for 24 h after which P-Akt-S473, P-Akt-T308, and total Akt were measured by Western blot. A: representative immunoblots of P-Akt-S473, P-Akt-T308, and total Akt. B: bar graph showing the changes in protein levels of P-Akt-S473 and P-Akt-T308. Values are means ± SE; n = 4. *P < 0.05 vs. empty vector.