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. 2016 Apr 20;311(1):C24–C34. doi: 10.1152/ajpcell.00295.2015

Fig. 5.

Fig. 5.

Calpain knockout (KO) inhibits Akt phosphorylation in smooth muscle of pulmonary arteries in mice with chronic hypoxic PAH. Five days after regimen of tamoxifen administration, control mice and ER-Cre+/−Capn4flox/flox mutant mice were exposed to room air (normoxia) or 10% oxygen (hypoxia) for 3 wk. Lung slides from ER-Cre+/−Capn4flox/flox mutant and control mice exposed to normoxia or hypoxia were double-stained for α-actin (red) and P-Akt-S473, P-Akt-T308, or total Akt (green). A: representative images of 8 independent experiments. B: bar graph depicting the changes of P-Akt-S473 and P-Akt-T308 in smooth muscle of pulmonary arteries. Values are means ± SE; n = 8. *P < 0.05 vs. normoxia in control mice. #P < 0.05 vs. chronic hypoxia in control mice.