Fig. 4.
SMS1 downregulation did not change channel voltage dependence or channel kinetics. A: conductance density-voltage relationship of a control cell transfected with scrambled RNA and a knockdown cell transfected with SMS1 shRNA. Boltzmann fit parameters are shown for each cell. B: box plots for maximum conductance density (gmax), half-activation voltage (Vh), and slope factor (Vc); open circles represent outliers. n = 24 (Control) or 24 (knockdown). *P < 0.05. C: currents evoked by a voltage step to 110 mV in 15 control cells, normalized to the maximum value. Thick line, mean; thin lines, mean ± 2 SD. D: difference current between the means of the control and knockdown groups (n = 15). Thick line, difference in mean currents; thin lines, ±2 SD for the control condition; the difference current during and after the voltage step never exceeded the 2 SD range, suggesting a lack of statistical difference between control and knockdown conditions.